Determination and Characterization of Thermostable Esterolytic Activity from a Novel Thermophilic Bacterium Anoxybacillus gonensis A4
نویسندگان
چکیده
منابع مشابه
Anoxybacillus gonensis sp. nov., a moderately thermophilic, xylose-utilizing, endospore-forming bacterium.
Seven closely related xylanolytic, thermophilic bacilli were isolated from mud and water samples from the Gonen and Diyadin hot springs, respectively located in the Turkish provinces of Balikesir and Agri. On the basis of morphology and biochemical characteristics, one of the isolates, designated strain G2(T), was studied further. Strain G2(T) is a xylanolytic, sporulating, Gram-positive, rod-s...
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α-Amylase from Anoxybacillus thermarum A4 was purified using ammonium sulphate precipitation and Sephadex G-100 gel filtration chromatography, with 29.8-fold purification and 74.6% yield. A4 amylase showed best performance for soluble potato starch hydrolysis at 70 °C and pH 5.5-10.5. A4 amylase was extremely stable at +4 °C, and the enzyme retained over 65% of its original α-amylase activity a...
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A gene (tap) encoding a thermostable alkaline phosphatase from the thermophilic bacterium Thermus thermophilus XM was cloned and sequenced. It is 1506 bp long and encodes a protein of 501 amino acid residues with a calculated molecular mass of 54.7 kDa. Comparison of the deduced amino acid sequence with other alkaline phosphatases showed that the regions in the vicinity of the phosphorylation s...
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# Corresponding author. Mailing address: Biotransformation and Biocatalysis, 18 Groningen Biomolecular Science and Biotechnology Institute, University of 19 Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands. E-mail: 20 [email protected]. Phone: +31-50-3634008; Fax: +31-50-3634165. 21 Copyright © 2013, American Society for Microbiology. All Rights Reserved. Appl. Environ. Microbiol. d...
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ژورنال
عنوان ژورنال: BMB Reports
سال: 2007
ISSN: 1976-6696
DOI: 10.5483/bmbrep.2007.40.4.588